Welcome!
The Wake Forest Institute for Regenerative Medicine (WFIRM) is a multidisciplinary lab dedicated to improving patient’s lives by developing regenerative medicine therapies and support technologies. The lab offers support through a wide range of sophisticated equipment and personnel in the areas of biomaterials, cell and tissue culture, histology, molecular, surgery and imaging (both in-vivo and in-vitro).
Imaging
The imaging suite has a wide range of imaging and microscopy resources available for researchers within the the department, the Innovation Quarter and users at large. Capabilities range from a quick look evaluation of slides and plates to multi-spectral, high resolution confocal imaging. Fluorescent viewing and live animal in-vivo image capabilities are available as well as the resources detailed below.
Flow Cytometry
The flow cytometry core consists of a BD FACSymphony A1 Cytometer. The core is responsible for training and scheduling of equipment, as well as maintenance.
Surgery Core
The surgery core provides surgical and anesthesia support services for pre-clinical studies held in the Richard Dean Building. The core is responsible for managing procedure room scheduling in support of WFIRM operations as well as equipment scheduling and maintainence.
Histology
The histology core provides fee-for-service tissue processing and embedding, as well as slide/cassette printing, sectioning, immunostaining and cryo-milling.
Tara Jones, Laboratory Manager
| Hours | Location |
|
Prime hours: Monday - Friday 7 am - 6:00 pm |
Wake Forest Institute for Regenerative Medicine |
| Service List |
| ► Biomaterials (7) | ||||
| Name | Description | Price | ||
|---|---|---|---|---|
| Syringe Pump Use | Inquire | |||
| High-performance liquid chromatography (HPLC) |
Location: Room 340 For inquiries, please contact Dr. Susan Sun xssun@wakehealth.edu |
Inquire | ||
| TA Instruments - Dynamic Mechanical Analyzer (DMA) |
Location: Room 340 For inquiries, please contact Dr. Susan Sun xssun@wakehealth.edu |
Inquire | ||
| Bruker Innova Atomic Force Microscope (AFM) |
Location: Room 342 For inquiries, please contact Dr. Susan Sun xssun@wakehealth.edu |
Inquire | ||
| Focus XCi Peptide Synthesizer |
Location: Room 340 Pricing is for trained users with no staff assistance. For inquiries, please contact Dr. Susan Sun xssun@wakehealth.edu |
Inquire | ||
| Focus XCi Peptide Synthesizer (with assistance) |
Location: Room 340 Pricing is including staff assistance. For inquiries, please contact Dr. Susan Sun xssun@wakehealth.edu |
Inquire | ||
| Freeze Dryer - Lyophilizer |
For assistance, please contact Ken Gyabaah kgyabaah@wakehealth.edu |
Inquire | ||
| ► Central Biorepository Shared Resource (CBSR) (13) | ||||
| Name | Description | Price | ||
| Aliquoting-Serum or Plasma |
Contact Kimberly Reeves at kreeves@wakehealth.edu for service request. |
Inquire | ||
| Blood Processing-Buffy Coat |
Contact Kimberly Reeves at kreeves@wakehealth.edu for service request. |
Inquire | ||
| Blood Processing-Plasma |
|
Inquire | ||
| Blood Processing-Plasma, WBC, and RBC from 10ml EDTA Tube |
|
Inquire | ||
| Blood Processing-Serum |
|
Inquire | ||
| DNA Integrity Analysis using TapeStation |
|
Inquire | ||
| DNA Isolation from Blood |
Contact Kimberly Reeves at kreeves@wakehealth.edu for service request. |
Inquire | ||
| DNA Isolation from PAXgene Blood DNA Tube |
Contact Kimberly Reeves at kreeves@wakehealth.edu for service request. |
Inquire | ||
| DNA/RNA Isolation from Cells & Tissues |
Contact Kimberly Reeves at kreeves@wakehealth.edu for service request. |
Inquire | ||
| DNA/RNA Purity Analysis using NanoDrop |
|
Inquire | ||
| DNA/RNA Quantification using Qubit |
Contact Kimberly Reeves at kreeves@wakehealth.edu for service request. |
Inquire | ||
| PBMC Isolation from CPT Tube (does not include cost of CPT tube) |
|
Inquire | ||
| RNA Integrity Analysis using TapeStation |
|
Inquire | ||
| ► Histology (20) | ||||
| Name | Description | Price | ||
| Antigen Retrieval |
Priced per rack. |
Inquire | ||
| Deparaffinization |
Priced per rack, up to 30 slides |
Inquire | ||
| Equipment Use- Cryostat |
Priced per hour. |
Inquire | ||
| Equipment Use- Microtome |
Priced per hour. |
Inquire | ||
| Extra Unstained slide | Inquire | |||
| Freeze and Section or Section Frozen block |
Up to 10 slides |
Inquire | ||
| H&E Staining, Automated |
Priced per rack, up to 30 slides. |
Inquire | ||
| Immunohistochemistry- Chromogen, Double Stain |
Price is per rack. |
Inquire | ||
| Immunohistochemistry- IF, Single stain |
Price is per rack. |
Inquire | ||
| Immunohistochemistry-Chromogen, Single Stain |
Price is per rack. |
Inquire | ||
| Immunohistochemistry-IF, Double Stain |
Price is per rack. |
Inquire | ||
| Paraffin Process , Embed and Section- up to 10 slides | Inquire | |||
| Paraffin Process and Embed |
Priced per block. |
Inquire | ||
| Paraffin Process Only | Inquire | |||
| Section Only | Inquire | |||
| Slide Printing |
Priced per slide |
Inquire | ||
| Special Stains- Tier 1 |
Price dependent on stain, per rack. |
Inquire | ||
| Special Stains- Tier 2 |
Price dependent on stain, per rack. |
Inquire | ||
| Special Stains- Tier 3 |
Price dependent on stain, per rack. |
Inquire | ||
| Special Stains- Tier 4 |
Price dependent on stain, per rack. |
Inquire | ||
| ► Microchip/Microfluidics (1) | ||||
| Name | Description | Price | ||
| Microfabrication |
The core provides various microfluidic platforms for customized applications. Some of the chips fabricated are application based perfusion system, bioreactor system, tumor-on-a-chip, organ-on-a-chip, multi-organ systems, droplet generator.
If you are making your own, please see information under Schedule Equipment.
Price: Pricing is customized for each order. Minimum $10/chip, but may vary based on complexity of chip
Availability (or time needed to complete): Minimum 2 weeks of advanced notice. Depending on the complexity of the chip and quantity required, might require additional time.
Customer Instructions for Reservation: The chip design can either be provided by the requestor or be designed by the fabrication core personnel. Please contact Shiny Rajan (shrajan@wakehealth.edu) for more information.
|
Inquire | ||
| ► Sequencing (24) | ||||
| Name | Description | Price | ||
| Bulk RNA Sequencing Analysis (1-16 samples) | Inquire | |||
| Bulk RNA Sequencing Analysis (17-32 samples) | Inquire | |||
| Bulk RNA Sequencing Analysis (33-48 samples) | Inquire | |||
| Bulk RNA Sequencing Analysis (49-64 samples) | Inquire | |||
| Bulk RNA Sequencing Analysis (65-80 samples) | Inquire | |||
| Bulk RNA Sequencing Analysis (81-96 samples) | Inquire | |||
| Sequencing @ 12.5M Reads/Sample, 100 Cycles (1-8 Samples) | Inquire | |||
| Sequencing @ 12.5M Reads/Sample, 100 Cycles (33-96 Samples) | Inquire | |||
| Sequencing @ 12.5M Reads/Sample, 100 Cycles (9-32 Samples) | Inquire | |||
| Sequencing @ 12.5M Reads/Sample, 100 Cycles (97-144 Samples) | Inquire | |||
| Sequencing @ 25M Reads/Sample, 100 Cycles (1-4 Samples) | Inquire | |||
| Sequencing @ 25M Reads/Sample, 100 Cycles (17-48 Samples) | Inquire | |||
| Sequencing @ 25M Reads/Sample, 100 Cycles (49-72 Samples) | Inquire | |||
| Sequencing @ 25M Reads/Sample, 100 Cycles (5-16 Samples) | Inquire | |||
| Sequencing @ 25M Reads/Sample, 100 Cycles (73-96 Samples) | Inquire | |||
| Sequencing @ 25M Reads/Sample, 300 Cycles (1-4 Samples) | Inquire | |||
| Sequencing @ 25M Reads/Sample, 300 Cycles (17-48 Samples) | Inquire | |||
| Sequencing @ 25M Reads/Sample, 300 Cycles (49-72 Samples) | Inquire | |||
| Sequencing @ 25M Reads/Sample, 300 Cycles (5-16 Samples) | Inquire | |||
| Sequencing @ 25M Reads/Sample, 300 Cycles (73-96 Samples) | Inquire | |||
| Single Cell RNA Sequencing Analysis (1-4 Samples) |
Cost to analyze 1-4 samples. |
Inquire | ||
| Single Cell Transcriptomics Library Preparation & Sequencing (1-4 Samples) |
Cost per run of 4 samples. |
Inquire | ||
| Stranded mRNA-Seq Library Preparation | Inquire | |||
| Stranded Total RNA-Seq Library Preparation | Inquire | |||
| ► Surgery (2) | ||||
| Name | Description | Price | ||
| Anesthesia Vaporstick - Add on for Room G062 | Inquire | |||
| OR Room Charge |
Internal
$24.00
each
|
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| ► Translational Core (6) | ||||
| Name | Description | Price | ||
| Vial of Bladder Smooth Muscle Cells (SMC) | Inquire | |||
| Vial of Penile Endothelial Cells (EC) | Inquire | |||
| Vial of Penile Smooth Muscle Cells (SMC) | Inquire | |||
| Vial of Placenta Stem Cells (PLS) Bead Selected | Inquire | |||
| Vial of Placenta Stem Cells (PLS) Tyto selected | Inquire | |||
| Vial of Urothelial Cells (UC) | Inquire | |||
| ► unclassified (1) | ||||
| Name | Description | Price | ||
| Nanostring Tech Time | Inquire | |||
