About

Cell Engineering Shared Resource

Overview

Overview

The mission of the Cell Engineering Shared Resource (CESR) is to serve the scientific needs of Wake Forest Baptist Comprehensive Cancer Center (WFBCCC) researchers. The CESR serves an essential function by providing investigators with considerable expertise in cell culture/cell line engineering/viral vector propagation, and the tools to analyse cell growth/death in vitro and in vivo under a wide variety of experimental conditions. The CESR is capable of designing, implementing and optimizing new cell culture/transduction procedures on behalf of investigators, and can conduct short-term experiments efficiently in a cost-effective manner. The CESR supports the research community at all of the Wake Forest Baptist Medical Center campuses, allowing investigators to make the most of their time and research dollars. The CESR also offers an on-site stockroom for tissue culture reagents and custom procurement services with substantial discounts from multiple vendors.

 

Research Project Support (contact Dr. Singh: rasingh@wakehealth.edu or Dr. Turner: jolturne@wakehealth.edu):

  1. customized assistance for cell culture/cell line engineering techniques including gene editing (CRISPR)/ and viral/plasmid vector transduction/ cell viability/growth analysis
  2. CRISPR design and optimization (in partnership with Sigma-Aldrich’s CRISPR Core Partnership Program)
  3. consultation and customized assistance to aid in design, implementation and optimization and trouble-shooting cell of new cell culture procedures
  4. production and concentration of replication-incompetent viruses including: lentivirus (ecotrophic and amphotrophic); AAV; adenovirus
  5. plasmid  expansion (tranformation; mini/midi/maxiprep; endotoxin free DNA purification)
  6. quality control and detailed records for each research project

 

Products and Services (contact Ms. Yelena Karpova: ykarpova@wakehealth.edu) or go to http://ccc.wfubmc.edu/corelab/serviceorder.aspx:

  1. on-site stockroom and distribution center with discounted pricing for cell culture media (including FBS), reagents and plastics (Fisher, ISCBioexpress); same day pick-up available
  2. daily ordering and significant discounts for non-stockroom products and services from external vendors including BioRad, eBioscience, Gemini, Lonza, LifeTech, PerkinElmer, Qiagen, Roche, Sigma
  3. free shipping/packaging/handling (including dry ice shipments) for all orders with distribution to all campuses daily
  4. ordering of primers from Invitrogen; orders placed daily
  5. Custom media and reagent preparation under strict quality control
  6. establishment of cells from a cell line repository (over 100 cancer cell lines)
  7. cryopreservation and storage of tissue culture cells (liquid nitrogen)
  8. mycoplasma testing and control

 

Equipment we support:

IVIS Lumina Series III

  • Non-invasive (fluorescence/luminescence) imaging of cells in rodents.

Essen BioSciences IncuCyte Zoom

  • Real-time, quantitative live-cell analysis

Biospherix Xvivo X3 Hypoxia Hood & Culture Comb (coming soon)

  • Growth of cells in low oxygen (hypoxic) conditions

 

 

Leadership

Ravi Singh, Ph.D

Department of Cancer Biology

rasingh@wakehhealth.edu

 

Staff

Yelena Karpova

ykarpova@wakehhealth.edu

Jane Strupe

jstrupe@wakehhealth.edu

Jolyn Turner, Ph.D

jolturne@wakehhealth.edu

 

Location and hours of operation

Hours     Location

Open:

9 am - 4 pm

Order pick-up:

9:30 am -11:30 am;

3 pm - 4 pm

   

    Medical Center Blvd

    Gray 2063

    Winston Salem, NC 27157

 

Links and Resources

  1. Cell and Viral Vector Core Laboratory

Contacts

Name Role Phone Email Location
Ravi Singh, PhD
Director
 
336-713-4434
 
rasingh@wakehealth.edu
 
Hanes 4045
 
Yelena Karpova
Core Staff
 
336-716-4280
 
ykarpova@wakehealth.edu
 
Gray 2063
 
Jane Strupe
Core Staff
 
336-716-4280
 
jstrupe@wakehealth.edu
 
Gray 2063
 
Jolyn Turner
Special Services
 
336-716-4280
 
joturne@wakehealth.edu
 
Gray 2063